Identifikasi Bakteri Escherichia Coli Dengan Pemeriksaan Kultur Dan Morfologi Di Taman Wisata Pemandian Air Panas Bora Sigi

IDENTIFICATION OF Escherichia coli BACTERIA USING CULTURE AND
MORPHOLOGICAL EXAMINATION AT BORA SIGI HOT SPRING
TOURISM AREA
Fadhilah Salsabilah A. Guli¹, M. Sabir², Ressy Dwiyanti³, Andi Nur Asrinawaty?
¹ Medical Study Program, Faculty of Medicine, Universitas Tadulako
² Department of Microbiology, Division of Tropical Infections and Traumatology, Faculty
of Medicine, Universitas Tadulako
³ Department of Microbiology, Faculty of Medicine, Universitas Tadulako
? Department of Microbiology, Faculty of Medicine, Universitas Tadulako
ABSTRACT
Background: Natural hot spring water is often considered sterile due to its high
temperature and mineral content, such as sulfur, which has antibacterial properties.
However, human activities and environmental conditions may lead to
microbiological contamination.
Objective: This study aimed to identify the presence of Escherichia coli bacteria in
the water of the Bora Hot Spring, Sigi Regency, using culture methods and
morphological examination.
Methods: This study employed a descriptive observational design. The study
population consisted of microorganisms present in the hot spring pool water at
Bora Hot Spring. Water samples were collected from three different pool points at
three different time intervals. Bacterial identification was carried out through
culture examination on nutrient agar using the pour plate method and microscopic
observation using Gram staining as the morphological identification instrument.
Results: The results showed that most samples were suspected to be positive for
Escherichia coli, particularly in the first and third pools. The colonies observed
were circular in shape with convex surfaces and smooth edges. Gram staining
revealed Gram-negative, short rod-shaped bacteria. Of the total 30 isolates
obtained, 7 isolates (23.33%) were suspected to be identified as Escherichia coli.
Conclusion: The Bora Sigi Hot Spring was suspected to be contaminated with
Escherichia coli bacteria.
Keywords: Bora Sigi Hot Spring, Escherichia coli, bacterial culture, Gram