IDENTIFIKASI BAKTERI Escherichia coliPADA ES DAWET DI AREAKOTA PALU DAN PEMANFAATANNYA SEBAGAI MEDIA PEMBELAJARAN

Escherichia coli is a Gram-negative bacterium from the family Enterobacteriaceae found in the human body and is used as an indicator
of hygiene in water, food, or beverages. The purpose of this study was to identify the presence of E. coli in ice dawet sold in the Palu City
area. This study used a descriptive-exploratory design with the Most Probable Number (MPN) method, Total Plate Count (TPC), and
biochemical testing using Triple Sugar Iron Agar (TSIA) medium. Samples were collected from five locations in the Palu City area using
simple random sampling. Based on the results, the Most Probable Number (MPN) test produced an average value of 18.700 MPN/mL of
sample, while the Total Plate Count (TPC) test showed an average value of 26.800 CFU/mL of sample, indicating that the samples did not
meet the standards set by the Indonesian National Standard (SNI) under the Ministry of Health, which states that the maximum allowable
microbial contamination in beverages is 0/100 mL. Confirmatory tests on Eosin Methylene Blue Agar showed colony growth with a
metallic green sheen and reddish coloration, characteristic of Gram-negative bacilli. The results of the Triple Sugar Iron Agar biochemical
test were positive, indicating biochemical characteristics consistent with E. coli. Therefore, the findings confirm the presence of E. coli in
the analyzed samples.
Keywords: Bacteria; Escherichia coli; Ice dawet.