IDENTIFIKASI BAKTERI Salmonella Sp. PADA GADO-GADO YANG D I JUAL AREA KAMPUS UNIVERSITAS TADULAKO DAN PEMANFAATANNYA SEBAGAI MEDIA PEMBELAJARAN

Salmonella spp. are Gram-negative rod-shaped bacteria commonly found in the gastrointestinal tract of humans and animals and are
recognized as one of the major causes of foodborne diseases. This study aimed to identify the presence of Salmonella spp. in gado-gado
sold in the canteens of Tadulako University. This research employed a descriptive exploratory laboratory design. Samples were collected
from five different canteens using a random sampling technique. Microbiological analyses were conducted through presumptive and
confirmatory tests using the Most Probable Number (MPN) method, Total Plate Count (TPC), Gram staining, cultivation on selective
Salmonella–Shigella Agar (SSA), and biochemical testing using Triple Sugar Iron Agar (TSIA). The MPN results showed an average
value of 4,900 MPN/mL of sample, while the TPC reached 35,800/ml sample. These values indicate that the food product does not meet
the microbiological safety standards established by the Indonesian Ministry of Health, as the TPC exceeds the maximum permissible limit
of 10? CFU/mL.