Analisis Filogenetika Diospyros Celebica Bakh. Berdasarkan Lokus TrnF-GAA Genome Kloroplas

ABSTRAK
Analisis filogenetika pada Eboni (Diospyros celebica Bakh), dilakukan untuk
melihat kekerabatan antar spesies maupun subspesies. Hal ini dilakukan dengan
cara identifikasi berbasis molekuler, menggunakan penanda lokus trnF-GAA.
Metode yang digunakan ialah experimental dan analisa in silico. Proses
experimental antara lain; Pengambilan sampel dilakukan pada dua lokasi diProvinsi
Sulawesi Tengah, yakni Maleyali Kecamatan Sausu dan Enu KecamatanSindue,
isolasi DNA, amplifikasi lokus trnF-GAA, pemurnian hasil PCR, elektroforesis
DNA dan perunutan/sekuensing lokus trnF-GAA. Analisa in silicodilakukan untuk,
analisis sekuens, BLAST NCBI, Pembuatan peta lokus trnF- GAA, pembuatan peta
circular genome lengkap Diospyros celebica dan rekonstruksi pohon filogenetik.
Dalam penelitian ini, dibuktikan bahwa lokus trnF-GAA dapat digunakan sebagai
penanda antar spesies dan belum bisa antar subspesies. Berdasarkan rekonstruksi
filogenetik letak Diospyros celebica dan sampel lainnya yang berbeda-beda, dapat
disimpulkan bahwa lokus trnF-GAA dapat dijadikan sebagai penanda biogeografi.
Kata kunci : Eboni (Diospyros celebica), trnF-GAA, DNA fingerprint, kloroplas
genome, analisis filogenetik

ABSTRACT
Phylogenetic analysis on Ebony (Diospyros celebica Bakh), was carried out to see
the relationship between species and subspecies. This was performed in a
molecularly based manner, using the trnF-GAA locus marker. The method used is
experimental and in silico analysis. Experimental processes include; Sampling was
carried out at two locations in Central Sulawesi Province, namely Maleyali, Sausu
District and Enu, Sindue District, DNA isolation, amplification of the trnF-GAA
locus, purification of PCR results, DNA electrophoresis and sequencing of the trnFGAA locus. In silico analysis was carried out for, sequence analysis, NCBI BLAST,
trnF-GAA locus mapping, complete circular genome mapping of Diospyros
celebica and phylogenetic tree reconstruction. In this study, it was proven that the
trnF-GAA locus can be used as a marker between species and not between
subspecies. Based on the phylogenetic reconstruction of the location of Diospyros
celebica and other different samples, it can be concluded that the trnF-GAA locus
can be used as a biogeographic marker.
Keywords: Ebony (Diospyros celebica), trnF-GAA, DNA fingerprint, chloroplast
genome, phylogenetic analysis