PENGARUH EKSTRAK TEMBULUK KELAPA MERAH (Cocos Nucifer Var. Rubescens)TERHADAP PENURUNAN KADAR KREATININ PADA MENCIT (Mus Musculus)

Abstract. Creatinine is a key biochemical indicator of renal function, and elevated creatinine levels reflect impaired
kidney performance. Carbon tetrachloride (CCl?) is a well-known nephrotoxic agent that induces oxidative stress
and renal damage. This study aimed to evaluate the effect of red coconut tembuluk (Cocos nucifera var. rubescens)
extract on reducing creatinine levels in CCl?-induced mice (Mus musculus). The study employed an experimental
laboratory design using 24 male mice, which were divided into six groups: normal control, negative control (CCl?-
induced), and four treatment groups administered red coconut tembuluk extract at concentrations of 10%, 20%,
40%, and 80%. Renal damage was induced by intraperitoneal injection of CCl? at a dose of 1 mL/kg body weight.
The extract was administered orally for eight consecutive days following induction. Serum creatinine levels were
measured before and after treatment. The results showed that CCl? induction significantly increased creatinine levels
in the negative control group compared to the normal control, indicating impaired renal function. Administration of
red coconut tembuluk extract resulted in a significant reduction in creatinine levels across all treatment groups, with
the most pronounced effect observed at the 80% concentration. Statistical analysis using one-way ANOVA
demonstrated a significant effect of the extract on creatinine reduction (p < 0.05), followed by a Least Significant
Difference (LSD) test confirming differences among treatment groups. The nephroprotective effect of the extract is
likely associated with its bioactive compounds, including flavonoids, antioxidants, and L-arginine, which contribute
to the reduction of oxidative stress and support renal tissue recovery. In conclusion, red coconut tembuluk extract
effectively reduces serum creatinine levels in CCl?-induced mice, with the 80% concentration showing the greatest
protective effect. These findings suggest that red coconut tembuluk has potential as a natural nephroprotective agent.